Electron microscope (EM) has revolutionized our ability to visualize the intricate details inside cells. The advancement to 3D electron microscopy, known as volume EM (vEM), has further expanded this three-dimensional, nanoscale imaging capacity. However, trade-offs between imaging speed, quality, and sample size still limit the achievable imaging area and volume. Concurrently, artificial intelligence (AI) is emerging as a pivotal force across various scientific domains, driving breakthroughs and serving as a vital tool in the scientific process.